From December 13 to December 19, the league reported 90 new positive cases, more than double the total positive tests in the previous week (42).
Prior to that, the league saw only 100 positive tests in the four months between August 2 and December 5.
The association says 77% of its players are currently vaccinated with two doses, which are considered fully vaccinated under current government guidance.
However, scientists have emphasized the importance of a third blow in the successful fight against Omicron.
The vaccination rate in England for those aged 25-29 is 78.5% – the average age of a Premier League player is 26.9 years.
The association said 84% of players are currently on a “vaccination journey” and should wait the required time between strikes.
The United Kingdom reported 91,743 new cases of Covid-19 on Monday, the second highest number since the start of the pandemic, according to government data. That’s up from 82,886 cases on Sunday.
The UK also reported 44 deaths within 28 days of testing positive for Covid-19 on Monday, bringing the death toll to 147,261 since the pandemic began.
The Premier League also announced on Monday that matches over the festive period will continue as planned despite a number of clubs experiencing Covid-19 outbreaks among players and staff – 10 matches have already been postponed this month as a result.
The decision was made after a virtual meeting of all 20 clubs and the league.
“The health and well-being of all involved remains our top priority and the Premier League will continue to monitor and reflect public health guidance, and will always proceed with caution,” a Premier League statement read.
“The League continues to work with clubs to encourage vaccination among players and club staff, as well as promoting governmental public health vaccination messages for clubs and the wider public.”
Premier League clubs are now operating under new Covid-19 protocols, which require them to follow rules such as wearing face coverings while indoors, observing social distancing and limiting treatment time.
The league has also increased lateral flow and PCR testing for players and staff.
Liverpool boss Jurgen Klopp has encouraged players and the wider community to get vaccinated and says people have a moral obligation to do so.
“It’s a matter of persuasion. I think it’s mandatory, from an ethical point of view, it should be mandatory for everyone I think, but not from a legal point of view,” he told reporters.
“I don’t see it but from an ethical point of view, because if I can do something that helps the people around me. Obviously people see it differently.”
Premier League club Wolverhampton Wanderers have also urged people to fully vaccinate.
The club on Monday tweeted a photo of coach Bruno Lage and captain Conor Coady getting a boost.
“Everyone in our building has been fully vaccinated, which is the right thing to do, especially with the increase in positive cases in the Premier League and the wider community,” said Lage.
Each of the Premier League teams is now scheduled to play three times between December 26 and January 3 in the busiest match period in the domestic league calendar.